Biotechnology

The H.Y.P. project

Humanized Yeast Project

The yeast: a system of choice

The yeast Saccharomyces cerevisiae is a particularly efficient system for the production of biomolecules, because of the following characteristics:

No time-consuming and cheap culture, in neutral culture media,

Large scale production, high yield,

Protein secretion in culture medium, easy purification,

Post- translational modifications.

Why performing humanized N-glycosylation?

Recombinant therapeutic proteins must have optimized properties, in terms of:

Structure,

Biological activity,

Half-life,

Targeting.

The nature of the glycans attached to a protein is determinant for the characteristics mentioned above (N-glycosylation).

How do we humanize yeast glycosylation?

Glycode has developed the GlycodExpress™ technology, which allows humanization of N-glycosylation in Saccharomyces cerevisiae. The main steps rely on:

Deletion of genes encoding enzymes responsible from hypermannosylation,

Genomic engineering of human-like N-glycosylation.

Those engineered strains are used for production of high quality recombinant glycoproteins with humanized complex glycans structures.

Scientific impacts

Using an innovative and powerful technology, the company provides a collection of yeast strains able to make an homogenous and humanized N-glycosylation. This allows to:

Optimize the efficiency of biomolecules, with a total control of their N-glycosylation (glycans homogeneity, site occupancy),

Produce biomolecules at low costs,

Provide to customers a solution to produce large amounts in a reduced time.